BAKIT BA KASI?????????????
HA!
Anong bang pakialam ng replication rate o copy number sa arabinose, ha?!
bakit ba masmadaming plasmid sa lb amp ara?!
o masmarami nga ba?
e bakit pa nagLB amp ara solution? bakit hindi lb amp?
GRABEEEEEEEEEEEEEEEEEEE
anyway, next question na nga muna :D wala kong naaachieve dito haha
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1 comment:
Hi Tin! :)
I hope the stress of the lab rep isn't affecting you too much.:) I'm sure if you look for the answers, you'd find them in time.:)
Anyway, I was very very frustrated with the same question too.:) But I made a quick search and I think I found the answer.:) pGLO has reporter genes including an ampicillin resistance gene and a GFP gene.:) But the GFP gene is only expressed when pGLO's ara gene is expressed in the presence of arabinose, making the colonies green.:)
But that doesn't answer your question.:) Colonies with pGLO plasmid can grow in the LB amp and LB amp/ara plates.:) And they'd grow at the same rate.:) It's just that the colonies in the LB amp/ara plate will be green under UV.:) I think it just gives you a super sure way of knowing the colony you have has the plasmid (although growing on the LB amp plate is already enough). So just to be super sure.:)
Here's my source of equal growth rates from Biorad: http://www.bio-rad.com/LifeScience/pdf/Biobusiness.pdf
God bless you Tin!:) Hope it works out.:) Yup, nakakastress talaga ang labrep.:)
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